Neurosphere Development from Hippocampal and Cortical Embryonic Mixed Primary
Neuron Culture: A Potential Platform for Screening Neuro-Chemical Modulator
Reconstitution of complex biological structure or system following simple and facile strategy using minimum physiochemical cues is challenging for in depth understanding of those systems. In particular, brain is a highly sophisticated and complex network of trillions of neurons and glial cells that controls function of our body. Understanding this complex machinery requires innovative and simple bottom-up approach. In this venture, we report an easy and efficient strategy to culture cortical and hippocampal primary neurons from the E14-E16 embryo of Sprague Dawley rat. This generates spontaneous neurospheres within 6-7 days of primary culture of E14-16 embryo. It further proliferates and forms radial glia like structures, which are known to be the primary neural progenitor cells that differentiate into neurons, astrocytes and oligodendrocytes. Interestingly, neurospheres leads to the formation of large projection neurons and radial glia, which mimic the early stage of cortical development in in vivo system. Overall, this new facile strategic mixed primary neuron culture method offers a potential platform for understanding the effect of neuro-chemical modulators, which has tremendous future implications in screening of neuro-therapeutics.